Douglas Costa and Danilo have been ruled out of Brazil's last World Cup group game against Serbia due to injury.
The duo will not travel with the rest of the squad for Wednesday's closing Group E clash in Moscow.
Danilo started Brazil's 1-1 opening draw against Switzerland but missed their 2-0 win over Costa Rica due to a hip problem, with Fagner deputising.
Costa was influential off the bench as late goals from Philippe Coutinho and Neymar downed Costa Rica on Friday.
But the Juventus winger will not be available for the Serbia match as he has a hamstring injury, team doctor Rodrigo Lasmar confirmed.
"At the end of yesterday's game, Douglas Costa complained of pain in his right hamstring," Lasmar told reporters. "Just remember he arrived injured, but in the left leg. That is different from this complaint.
"He was diagnosed with a minor muscle injury so he will not travel with us. He will stay in Sochi, where we have a structure suitable for him to recover as soon as possible.
"We'll have a physiotherapist here. He will continue the recovery process here, along with Danilo, who will not travel and will have physiotherapy aimed at recovery.
"We are optimistic about his participation in the competition, depending on our performance at the World Cup. We are sparing no effort so can he still help us.
"The case that is most worrying is Douglas Costa's, but Danilo's is not much different, except that his is a less important, secondary muscle, so there's a little better expectation with respect to him."
Lasmar, though, is not willing to speculate on when Costa may be ready to return to action, with Brazil needing a point against Serbia to guarantee a last-16 spot.
"We are not going to need a date when he will be ready," Costa said.
"It is clear he is not in condition for this game and his recovery will tell us. We are imagining within a favourable time in the competition."
|Steelers´ Roethlisberger texted Bell but ´didn’t hear back´|
|Reus and Kroos to sit out Germany´s clash with Russia|
|Van Dijk happy to recommend De Ligt if Liverpool ask|
|Sauber announce launch of junior programme|
|No ´breathtaking tennis´ in Djokovic´s win against Zverev|
|Curran: England spinners will be key in Kandy|
|Djokovic charges past Zverev to move closer to ATP Finals progression|
|Messi is a football alien - Malcom|
|Sky´s the limit for Sancho, says Dortmund team-mate Pulisic|
|Radwanska retires from tennis at 29|
|Maybe one day - Pulisic open to Premier League move|
|Suso ´proud´ of Real Madrid speculation but happy at AC Milan|
|Betis president convinced Lo Celso will stay|
|Injured Holder out of Bangladesh tour|
|Germany face relegation, final spots up for grabs - Nations League state of play|
|James Rodriguez out for several weeks with knee injury|
|Curran´s brilliant fifty rescues England´s innings|
|Lenglet: No problem with Dembele at Barca|
|Townsend rings the changes for Springboks clash|
|Claudio Ranieri´s managerial career in Opta numbers|
|Mahmudullah´s first century in eight years boosts Bangladesh|
|Fellaini gets the chop ahead of 31st birthday|
|Maradona: Solari will not last at Real Madrid|
|Molinari: Race to Dubai success would not surpass Ryder Cup glory with Fleetwood|
|Dortmund star Sancho was a Bayern target, confirms Salihamidzic|
|Gerrard enjoying ´buzz´ of management|
|Hamilton questions approach to Formula One expansion|
|Ranieri honoured to lead ´exceptional´ Fulham squad|
|Solari saw Isco out of shape - Ceballos|
|Ranieri replaces Jokanovic at Fulham|
|Durant keeps details of Green spat ´in-house´ but envisages resolution|
|Rooney: Kane and Sterling are England superstars|
|Messi will make Argentina return, says Maradona|
|Warriors drop Hawks as Green serves suspension|
|In-form Healy sends Australia into semis|
|Former NBA star McGrady says Carmelo Anthony should retire|
|Celtics´ Smart: We have to look at ourselves in the mirror|
|Federer plays down preferential treatment talk|
|Mourinho is better than Guardiola – Maradona|
|Mbappe plays down Pele comparisons|
|Don´t worry bro – Mendy responds to Trump|
|Melvin, Snitker win manager of the year awards|
|Warriors´ Green suspended for Hawks clash|
|Asensio: It´s not for me to lead Madrid|
|Rooney ready to enjoy England farewell after pressure of ´Golden Generation´|
|Ludwigsburg stun high-flying Murcia in BCL|
|Rams v Chiefs moved to LA due to Mexico City field conditions|
|Skriniar discussing new Inter contract, confirms agent|
|Former Barcelona boss Martino named MLS Coach of the Year|
|Bell fails to report to Steelers, will sit out rest of season|
|Federer back on track after dismal Thiem display|
|Udinese appoint Nicola after sacking Velazquez|
|Monaco deny they broke rules regarding youth recruitment|
|Del Bosque, Zidane, Lopetegui - The former Castilla coaches Solari follows into Real Madrid hot seat|
|Ozil rejected ´crazy offers´ from Asia, reveals agent|
|Real Madrid appoint Solari on permanent basis|
|LeVert avoids need for surgery, should return this season|
|Neuer confident despite concerns over Bayern Munich form|
|All I ever wanted to do is win, says Sixers signing Butler|
|Pogba: Coming home to United was the best feeling ever|
|Watson among four signing new Bath deals|
|Susanna Dinnage to replace Scudamore as Premier League chief executive|
|Murray commits to Brighton until 2020|
|Rooney rejected chance of pre-World Cup farewell|
|Impressive Anderson thrashes Nishikori|
|Ozil´s Germany absence a ´pity´, says Sane|
|Rooney: I´ll finish my career in the US|
|McIlroy unsure of 2019 European Tour membership|
|Australian Open dismisses concerns of Federer scheduling bias|
|Hue Jackson joins Bengals staff as ´special assistant´|